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Table 2 Samples from P. californicus either negative control (un) or infected with volepox virus (inf) were tested with G9R-NA OPXV rt-PCR assay

From: Detection of North American orthopoxviruses by real time-PCR

Specimen Day 6 Day 7 Day 8 Total No. of specimens
  (Inf) (un) (inf) (un) (inf) (un) (inf) (un)
Blood 4 1 3 1 2 1 9 3
Oral swab 6 1 5 1 7 1 18 3
Ocular swab 6 1 8 1 2 1 13 3
Anal swab 5~ 1 3~ 1 2 1 13 3
Spleen 6 1 5 1 2 1 13 3
Gonad 6 1 5 1 2 1 13 3
Kidney 6 1 5 1 2 1 13 3
Lymph node 6 1 5 1 2 1 13 3
Supradrenal 6 1 4 1 2 1 12 3
Brain 6 1 5 1 2 1 14 3
Liver 6 1 5 1 2 1 14 3
Lung 6 1 5 1 2 1 14 3
Skin lesion 5 1 2 1 10 1 14 3
Tognue lesion 5 1 2 1 NA 1 7 3
Intestine lesion NA 1 2 1 NA 1 2 3
Bladder NA NA 2 NA 1 NA 3 NA
Pancreas NA NA 1 NA 1 NA 2 NA
Number of positive/tested 79/80 0/15 64/66 0/15 41/41 0/15 184/187 0/45
  1. ~DNA was detected in all samples from infected animals (inf) but 3 anal swabs (1 from Day 6 and 2 from Day 7).
  2. DNA was not detected in any negative control (un). NA, not applicable.