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Table 2 Samples from P. californicus either negative control (un) or infected with volepox virus (inf) were tested with G9R-NA OPXV rt-PCR assay

From: Detection of North American orthopoxviruses by real time-PCR

Specimen

Day 6

Day 7

Day 8

Total No. of specimens

 

(Inf)

(un)

(inf)

(un)

(inf)

(un)

(inf)

(un)

Blood

4

1

3

1

2

1

9

3

Oral swab

6

1

5

1

7

1

18

3

Ocular swab

6

1

8

1

2

1

13

3

Anal swab

5~

1

3~

1

2

1

13

3

Spleen

6

1

5

1

2

1

13

3

Gonad

6

1

5

1

2

1

13

3

Kidney

6

1

5

1

2

1

13

3

Lymph node

6

1

5

1

2

1

13

3

Supradrenal

6

1

4

1

2

1

12

3

Brain

6

1

5

1

2

1

14

3

Liver

6

1

5

1

2

1

14

3

Lung

6

1

5

1

2

1

14

3

Skin lesion

5

1

2

1

10

1

14

3

Tognue lesion

5

1

2

1

NA

1

7

3

Intestine lesion

NA

1

2

1

NA

1

2

3

Bladder

NA

NA

2

NA

1

NA

3

NA

Pancreas

NA

NA

1

NA

1

NA

2

NA

Number of positive/tested

79/80

0/15

64/66

0/15

41/41

0/15

184/187

0/45

  1. ~DNA was detected in all samples from infected animals (inf) but 3 anal swabs (1 from Day 6 and 2 from Day 7).
  2. DNA was not detected in any negative control (un). NA, not applicable.