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Table 1 Panel of viral DNA used to validate the G9R-NA OPXV rt-PCR assay

From: Detection of North American orthopoxviruses by real time-PCR

Old World viruses

Sample ID

Accession No.

Ortholog

Viral Prep.

2 ng*

Camelpox

CMLV-V78 I

Not available

Not available

Pure

ND

Cowpox

CPXV-Sweden

Not available

Not available

Pure

ND

Ectromelia

ECTV-MOS

NC_004105

73

Pure

ND

Monkeypox

MPXV-USA

DQ011157

84

Pure

ND

Orf

ORFV-NZ2

DQ184476

44

Pure

ND

Squirrel Fibroma

SQFV-Kilham

Not available

59

Crude

ND

Tanapox

TAV-Kenya

NC_009888

63

Pure

ND

Taterapox

TATV-DAH68

NC_008291

90

Pure

ND

Vaccinia

VACV-COP

M35027

108

Pure

ND

Variola

VARV-BSH

L22579

76

Pure

ND

North American viruses

Raccoonpox

RCNV-1964

FJ807746-54

70

Pure

17

Skunkpox

SKPV-1978

FJ807755-63

86

Pure

17

Volepox

VPXV-1985

FJ807737-45

86

Pure

16

  1. *The average of the cycle when fluorescence crossed the threshold (Ct) for each positive sample is shown. ND, not detected.