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Table 2 Oligonucleotide primers used for genotyping

From: Novel norovirus recombinants and GII.4 sub-lineages associated with outbreaks between 2006 and 2010 in Belgium

Region

Name

Sequence (5' → 3')a

Sense

Positionb

Reference

A

290d

GATTACTCCASSTGGGAYTCMAC

+

4568-4590

[50]

A

289d

TGACGATTTCATCATCMCCRTA

-

4865-4886

 

A

JV12Y

ATACCACTATGATGCAGAYTA

+

4552-4572

[7]

A

JV13I

TCATCATCACCATAGAAIGAG

-

4858-4878

 

C

G1SKF

CTGCCCGAATTYGTAAATGA

+

5342-5361

[26]

C

G1SKR

CCAACCCARCCATTRTACA

-

5652-5671

 

C

G2SKF

CNTGGGAGGGCGATCGCAA

+

5046-5064c

 

C

G2SKR

CCRCCNGCATRHCCRTTRTACAT

-

5367-5389c

 

Capsid

FW1

GCGATCGCAATCTGGCTCCCAG

+

5055-5076d

[27]

Capsid

RT5

AGGTGYACATTATGACCAGTTC

-

6795-6819d

 
  1. a International Union of Biochemistry ambiguity codes: I, inosine; N, (A/C/T/G); H, (A/C/T); Y, (C/T); M, (A/C); R, (A/G); S, (C/G).
  2. b Position in the norovirus genomic sequence GI.1 (M87661)
  3. c Position in the norovirus genomic sequence GII.1 (U07611)
  4. d Position in the norovirus genomic sequence GII.4 (AY032605)
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