Table 5 Single-dose 2mH9N2:6WF10att in-ovo vaccination study in chickens challenged with low-pathogenic H9N2 (WF10) at 2 and 6 weeks post-hatching
Vaccine dose (EID50)/embryo age (days) | # positive HI/total # before challenge | Age (in weeks) at time of challenge | # Shedding virus/total # in swabs (log10TCID50/ml ± SD) | |||
|---|---|---|---|---|---|---|
Tracheal | # positive HI/total # at 14 dpi | |||||
3 dpc | 5 dpc | 7 dpc | ||||
0 (Mock) | 0/7 | 2 | 7/7 (2.7 ± 0.6) | 7/7 (2.5 ± 0.3) | 0/7 | 7/7 (217) |
106, 18 | 3/5 (14) | 2 | 2/5 (2.2 ± 0.2) | 2/5 (2.3 ± 0.4) | 0/5 | 5/5 (192) |
10 6 , 19 | 6/7 (32) | 2 | 1/7 (2.5) | 0/7 | 0/7 | 7/7 (286) |
107, 19 | 3/4 (32) | 2 | 1/4 (2.3) | 0/4 | 0/4 | 4/4 (260) |
0 (Mock) | 0/7 | 6 | 7/7 (2.5 ± 0.3) | 7/7 (2.5 ± 0.2) | 0/7 | 7/7 (320) |
106, 18 | 2/5 (30) | 6 | 3/5 (2.6 ± 0.7) | 3/5 (2.2 ± 0.9) | 0/5 | 5/5 (224) |
10 6 , 19 | 5/7 (71) | 6 | 2/7 (2.4 ± 0.5) | 0/7 | 0/7 | 7/7 (446) |
107, 19 | 3/3 (67) | 6 | 0/3 | 0/3 | 0/3 | 3/3 (227) |