Table 4 Single-dose 2mH7N2:6WF10att in-ovo vaccination study in chickens challenged with low-pathogenic H7N2 (Ck/04) at 2 and 6 weeks post-hatching
Vaccine dose (EID50)/embryo age (days) | # positive HI/total # pre-challenge (HI titer) | Age (in weeks) at time of challenge | # Shedding virus/total # in swabs (log10TCID50/ml ± SD) | # positive HI/total # at 14 dpi | |||||
|---|---|---|---|---|---|---|---|---|---|
Tracheal | Cloacal | ||||||||
3 dpc | 5 dpc | 7 dpc | 3 dpc | 5 dpc | 7 dpc | ||||
0 (Mock) | 0/8 | 2 | 8/8 (3.4 ± 0.8) | 8/8 (2.9 ± 0.6) | 0/8 | 2/8 (3.7) | 5/8 (3.4 ± 0.2) | 5/8 (3.2 ± 0.5) | 8/8 (170) |
106, 18 | 1/4 (3) | 2 | 3/4 (3.3 ± 1.0) | 3/4 (2.9 ± 0.9) | 0/4 | 2/4 (4.5 ± 0.7) | 3/4 (3.7 ± 1.0) | 3/4 (3.7 ± 0.7) | 4/4 (320) |
10 6 , 19 | 6/8 (13) | 2 | 2/8 (3.5 ± 0.7) | 1/8 (2.3) | 0/8 | 0/8 | 0/8 | 1/8 (2.0) | 8/8 (240) |
107, 19 | 3/5 (5) | 2 | 1/5 (2.7) | 0/5 | 0/5 | 0/5 | 0/5 | 1/5 (2.3) | 5/5 (272) |
0 (Mock) | 0/7 | 6 | 7/7 (3.5 ± 0.7) | 7/7 (3.4 ± 0.7) | 0/7 | 3/7 (3.9 ± 0.5) | 5/7 (3.7 ± 1.0) | 5/7 (3.3 ± 0.8) | 7/7 (525) |
106, 18 | 2/4 (50) | 6 | 2/4 (4.1 ± 0.6) | 2/4 (3.9 ± 0.6) | 0/4 | 1/4 (3.5) | 2/4 (4.3 ± 0.4) | 2/4 (3.6 ± 0.1) | 4/4 (360) |
10 6 , 19 | 5/7 (51) | 6 | 2/7 (3.4 ± 0.2) | 0/7 | 0/7 | 1/7 (3.7) | 1/7 (3.5) | 1/7 (3.3) | 7/7 (525) |
107, 19 | 4/5 (64) | 6 | 1/5 (3.7) | 1/5 (3.7) | 0/5 | 0/5 | 0/5 | 0/5 | 5/5 (640) |