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Table 2 Comparison of the hatchability of new recombinant viruses in embryonated chicken eggs vs. the viruses with wild type HAs and the optimization of the dose and timing for in-ovo vaccination.

From: Improved hatchability and efficient protection after in ovo vaccination with live-attenuated H7N2 and H9N2 avian influenza viruses

Vaccine

Dose (EID50)

Embryo age (Day)

% Hatchability (# hatched/total #)

H7N2 (VIVA/04) Vaccine

2mH7N2:6WF10 att

106

18

50% (15/30)

    

(P = 0.016)

  

106

19

93% (42/45)

    

(P = 0.061)

  

107

19

80% (24/30)

    

(P = 0.066)

 

2H7N2:6WF10att

106

18

30% (9/30)

  

106

19

43% (13/30)

  

107

19

37% (11/30)

H9N2 (WF10) Vaccine

2mH9N2:6WF10att

106

18

63% (19/30)

    

(P = 0.0161)

  

106

19

90% (27/30)

    

(P = 0.260)

  

107

19

83% (25/30)

    

(P = 0.154)

 

2H9N2:6WF10att

106

18

37% (11/30)

  

106

19

60% (18/30)

  

107

19

37% (11/30)

PBS (Mock)

0

18

93% (28/30)

  

0

19

96% (43/45)

  1. * 3 chickens dead at 2-5 days post-hatching.