Figure 3
From: Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
Expression levels of the candidate reference genes. (A) Expression levels displayed as cycle threshold (Ct) values of the genes used in this study, represented in a box and whisker diagram. Whiskers represent the maximum and minimum values. The genes were ordered from the least (higher Ct, to the left) to the most abundantly expressed (lower Ct, to the right). (B) Comparison of the expression levels of candidate reference genes in naïve versus MRCV-infective planthoppers. Differences between treatments were statistically evaluated for each gene by the Mann-Whitney t test.