Analysis of VP2 gene expression. (A) Western blot analysis of VP2 protein expressed by infected Vero cells. Lanes: (M) Pre-stained protein markers, (1) Vector PRV SA215; (2) Recombinant PRV SA215/VP2. The 93-kDa band represents EGFP and VP2 fusion protein. (B and C) Indirect immunofluorescence analysis of VP2 protein expressed in Vero cells infected with recombinant PRV SA215/VP2 (B) and vector PRV SA215 (C). Cells infected with recombinant PRV SA215/VP2 developed immunofluorescence (B), and cells infected with vector virus PRV SA215, did not develop immunofluorescence (C).