In vivo cytotoxicity assays to determine the specific lytic potential of effector CD8+ T cells in mice immunised with rVVJ16/18E7E6. (A) Target cells were transferred into mice (n = 3 per group) immunised with virus rVVJ16/18E7E6 or virus rVVJ1175 or into naïve mice. Numbers represent the percentage of target cells killed. (B) The bar graph indicates the percentage of specific killing for peptides HPV16E749-57- and HPV18E667-75 -pulsed targets from the indicated groups of mice. Data are given as mean ± SD of two independent experiments. The percentage of specific killing was calculated as follows: 100-[(% peptide pulsed in infected/% unpulsed in infected)/(% peptide pulsed in uninfected/% unpulsed in uninfected)] × 100. Asterisks represent statistically significant differences relative to the rVVJ1175 virus control (* p < 0.05, ** p < 0.001).