Figure 4From: Immunogenicity in mice and rhesus monkeys vaccinated with recombinant vaccinia virus expressing bivalent E7E6 fusion proteins from human papillomavirus types 16 and 18In vivo cytotoxicity assays to determine the specific lytic potential of effector CD8+ T cells in mice immunised with rVVJ16/18E7E6. (A) Target cells were transferred into mice (n = 3 per group) immunised with virus rVVJ16/18E7E6 or virus rVVJ1175 or into naïve mice. Numbers represent the percentage of target cells killed. (B) The bar graph indicates the percentage of specific killing for peptides HPV16E749-57- and HPV18E667-75 -pulsed targets from the indicated groups of mice. Data are given as mean ± SD of two independent experiments. The percentage of specific killing was calculated as follows: 100-[(% peptide pulsed in infected/% unpulsed in infected)/(% peptide pulsed in uninfected/% unpulsed in uninfected)] × 100. Asterisks represent statistically significant differences relative to the rVVJ1175 virus control (* p < 0.05, ** p < 0.001).Back to article page