Figure 3From: Immunogenicity in mice and rhesus monkeys vaccinated with recombinant vaccinia virus expressing bivalent E7E6 fusion proteins from human papillomavirus types 16 and 18Intracellular cytokine staining with flow cytometry analysis to determine the proportion of specific IFN-γ -expressing CD8+ T cells. Splenocytes from mice (n = 3 per group) immunised with virus rVVJ16/18E7E6 and virus rVVJ1175 were cultured and stimulated with either HPV16E749-57 or HPV18E667-75 peptide. Splenocytes without peptide stimulation were used as a negative control. The splenocytes were stained for both CD8+ and intracellular IFN-γ (A) Representative intracellular cytokine staining. The number of CD8+ IFN-γ + T cells in 1 × 106 splenocytes are indicated in the upper right corner. (B) Bar graph depicting the number of specific IFN-γ expressing CD8+ T cells per 1 × 106 splenocytes (mean ± SD) following in vitro stimulation in two independent experiments. Asterisks represent statistically significant differences relative to the rVVJ1175 virus control (* p < 0.05, ** p < 0.001).Back to article page