Production of NO while NS1 is bound to PSD-95 in hippocampal neurons. Hippocampal neurons were cultured for 3 days then infected by NS51-lentivirus or the control virus pGC FU-GFP-LV. At 3 days post-infection, the cells were treated with Ach for 24 h and the release of NO was measured. Data were from 3 independent experiments. Relevant levels of significance are illustrated in the figure as ** for p < 0.01. NS51-lentivirus and pGC FU-GFP-LV were used as positive and negative controls, respectively.