Differential binding of different NS1 proteins with PSD-95 in vitro. (A) GST or GST-PSD-95 proteins were expressed in E. coli BL21 and immobilised on magnetic beads. The immobilised GST or GST-PSD-95 proteins were separated in 8% SDS-PAGE gel and detected by Coomassie blue staining. M stands for molecular weight standard. (B) NS11 or NS51 were translated in vitro using plasmids pcDNA3-NS11 or pcDNA3-NS51 and the TNT T7 Quick Coupled Transcription/Translation Systems. Expression of NS11 and NS51 was comfirmed by western blotting using anti-NS1 antibody. (C) NS11 or NS51 was incubated with GST or GST-PSD-95 immobilised onto magnetic beads. After extensive washing, bound proteins were dissociated from beads and subjected to western blotting analysis with anti-NS1 antibody.