Figure 2

Protein expression analysis of HCV E2 gene and HCV receptors genes by using siRNA alone and in combination against HCV gene E2 and HCV receptor genes CD81, LDLR. Protein isolated from Huh-7 cells treated with single and combination of siRNA against HCV E2 gene and receptor CD81 and LDLR genes and incubated for 6 hrs before adding HCV-3a sera (Ser 3a) for 48 hrs. Protein levels were quantified by western blot analysis using specific antibodies of CD81, LDLR, E2 and GAPDH. A) Silencing of CD81 gene and HCV 3a E2 gene alone and in combination using specific antibodies showing reduction at protein expression level. B) Silencing of LDLR gene and HCV 3a E2 gene alone and in combination, using specific antibodies showing reduction at protein expression level. Protein levels for GAPDH gene are also shown as internal control and scramble siRNA (Sc) as siRNA control.