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Table 2 Primer sequences

From: Effective inhibition of foot-and-mouth disease virus (FMDV) replication in vitro by vector-delivered microRNAs targeting the 3D gene

Primer

Sequence

Target gene

Size

Purpose

3DF

5'-TGCGGTACC ATGGGGTTGATTGTCGACACCA-3'

3D

1.4kb

Gene cloning

3DR

5'-GAGTCTAGA TGCGTCACCGCACACGGCGTTC-3'

   

3D1F

5'-ACTGGGTTTTACAAACCTGTGA-3'

3D

107bp

Real-time PCR

3D1R

5'-GCGAGTCCTGCCACGGA-3'

   

GHF

5'-GGCAAGTTCAAAGGCACAGTC-3'

GAPDH

115bp

Real-time PCR

GHR

5'-CACCAGCATCACCCCATTT-3'

   
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Virology Journal

ISSN: 1743-422X