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Table 2 Primer sequences

From: Effective inhibition of foot-and-mouth disease virus (FMDV) replication in vitro by vector-delivered microRNAs targeting the 3D gene

Primer Sequence Target gene Size Purpose
3DF 5'-TGCGGTACC ATGGGGTTGATTGTCGACACCA-3' 3D 1.4kb Gene cloning
3DR 5'-GAGTCTAGA TGCGTCACCGCACACGGCGTTC-3'    
3D1F 5'-ACTGGGTTTTACAAACCTGTGA-3' 3D 107bp Real-time PCR
3D1R 5'-GCGAGTCCTGCCACGGA-3'    
GHF 5'-GGCAAGTTCAAAGGCACAGTC-3' GAPDH 115bp Real-time PCR
GHR 5'-CACCAGCATCACCCCATTT-3'