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Figure 7 | Virology Journal

Figure 7

From: First construction of infectious clone for newly emerging mutation porcine circovirus type 2 (PCV2) followed by comparison with PCV2a and PCV2b genotypes in biological characteristics in vitro

Figure 7

PCR-RFLP profiles of the amplified rescued PCV2 genomic DNA after digestion with restriction enzyme of Fba I (A) and Acc I (B). Rescued names are indicated above the corresponding lanes. Panel A shows the Fba I digest of the PCR amplification fragments and restriction fragments are evident at 1165 and 602 bp for PCV2b/rJF, the DNA of other rescued strains (PCV2a/rCL, PCV2b/rYJ and PCV2d/rBDH) remains undigested. Panel B shows the Acc I digest of the PCR amplification fragments and restriction fragments are evident at 1257 and 511 bp for PCV2a/rCL; 1532 and 235 bp for PCV2d/rBDH; and 1021 511 and 235 bp for PCV2b/rJF and PCV2b/rYJ. The four rescued strains of different genotypes as well as mutation rescued viruses can be differentiated by the method via combination use of Fba I and Acc I.

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