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Table 1 PCR condistions and Primers

From: Characterization of herpes simplex virus clinical isolate Y3369 as a glycoprotein G variant and its bearing on virus typing

Primer Sequence
UL1 for 5'-GAGACCCCCTCGGCTATAAA-3'
UL1 rev 5'-CGTTTCTGTTTCCTGGGTGT-3'
UL10 for 5'-GAGCCTTGTGGGCACTTATG-3'
UL10 rev 5'-GTGATCTGCAGCAACCAAGA-3'
UL 22 for 5'-AAACAAAAGCGCTCCTCGTA-3'
UL22 rev 5'-GACAGACCCATGGTTTTTGG-3'
glycoprotein G for 5'-GCTGTTTGCGGGTTGGCACA-3'
glycoprotein G rev 5'-TCCCCCGCCCCATACCCTAC-3'
glycoprotein D for 5'-TTTGTGTGGTGCGTTCCGGT-3'
glycoprotein D rev 5'-TCCCATCCCAACCCCGCAGA-3'
  1. PCR conditions for all reactions:
  2. 94°C 2 min, 40 cycles of [94°C 15 s, 57°C 15 s, 72°C 1:15], 72°C 2 min