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Table 1 Primer sequences used in the construction of the viral UTR-driven Gluc reporter

From: A reporter system for assaying influenza virus RNP functionality based on secreted Gaussia luciferase activity

Gluc-F

5'-GGCCGCCGGGTTATT AGTAGAAACAAGGGTATTTTTCT TTAGTCACCACCGGCCCCCTTGATC -3'

Gluc-R1

5'-CCTCCGAAGTTGGGG AGCAAAAGCAGGGTAGATAATCACTCACAGAGTGACATCGAAA AT-3 '

Gluc-R2

5'-CACAGAGTGACATCGAAA ATGGGAGTCAAAGTTCTGTTTGCCCT-3'

  1. Bold portions correspond to sequences within the coding region of the reporter gene. The remaining 5' sequences (15 nt, italics) are necessary for In-Fusion cloning into the RNA polymerase I vector polI-sapI. Underlined parts correspond to the untranslated regions of the A/WSN/33 NP segment. Two reverse primers (R1, R2) were designed to ensure PCR efficiency.
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Virology Journal

ISSN: 1743-422X