Figure 4
RNP activity of QH virus containing PB2-627K and PB2-627E. Fluc (A) and Gluc (signaling was detected after 1:10 dilution; B) were expressed from a synthetic viral RNA respectively when the influenza virus polymerase complex was present. 293T cells were transfected in 96- (Gluc; 5 × 104/well) and 24-well (Fluc; 1 × 105/well) plates. Control cells were mock transfected with the reporter plasmid alone. Data are expressed as standard errors of the mean (SEM) from three independent experiments performed in triplicate. * P < 0.05.