Figure 3

The effect of virus dose and addition of antivirals on RNP activity. A549 cells were transfected with plasmids pYH-Fluc (A) or polI-Gluc (B, C, and D) and incubated with or without amantadine (0.21 μM) for 30 min at 6 h post-transfection. Infections were performed with recombinant viruses G1 (A, B), QH (PB2-627K) (C), or QH (PB2-627E) (D) at a MOI of 1 and 0.001. At 24 h p.i., cells were lysed, and Fluc (A) and Gluc activity (B-D) were assayed. Data are expressed as standard errors of the mean (SEM) from three independent experiments performed in triplicate. A, amantadine; * P < 0.05.