Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype

Table 2 Dengue virus deletion mutations produced in the first TMD of the M protein of DV2

SEQUENCE M-T1 DOMAIN			Foci		Critical AA Vero	Critical AA C6/36
			Vero	C6/36
	WT DV2	₂₄₅ PGFTMMAAILAYTIG ₂₅₉	++	++
1	ΔA₂₅₁	PGFTMM-AILAYTIG	+	+
2	ΔAA_251-52	PGFTMM--ILAYTIG	-	-	AA_251-52	AA_251-52
3	ΔAAI_251-53	PGFTMM---LAYTIG	-	-	AAI_251-53	AAI_251-53
4	ΔAAIL_251-54	PGFTMM----AYTIG	+	+
5	ΔAAILA_251-55	PGFTMM-----YTIG	-	-	5aa	5aa
6	ΔM₂₅₀	PGFTM-AAILAYTIG	+	+
7	ΔMM_249-50	PGFT--AAILAYTIG	+	-		M₂₄₉
8	ΔTMM_248-50	PGF---AAILAYTIG	+	-		M₂₄₉
9	ΔFTMM_247-50	PG----AAILAYTIG	-	-	5aa	5aa

The primary DV2 mutant focus assay screen determined which mutants were able to produce foci of infections in Vero and A. Albopictus C6/36 cells. (+) indicates the presence of foci and (-) indicates no foci were observed. AA; amino acid.

ISSN: 1743-422X