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Table 2 Dengue virus deletion mutations produced in the first TMD of the M protein of DV2

From: Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype

SEQUENCE M-T1 DOMAIN Foci Critical AA Vero Critical AA C6/36
    Vero C6/36   
  WT DV2 245 PGFTMMAAILAYTIG 259 ++ ++   
1 ΔA251 PGFTMM-AILAYTIG + +   
2 ΔAA251-52 PGFTMM--ILAYTIG - - AA251-52 AA251-52
3 ΔAAI251-53 PGFTMM---LAYTIG - - AAI251-53 AAI251-53
4 ΔAAIL251-54 PGFTMM----AYTIG + +   
5 ΔAAILA251-55 PGFTMM-----YTIG - - 5aa 5aa
6 ΔM250 PGFTM-AAILAYTIG + +   
7 ΔMM249-50 PGFT--AAILAYTIG + -   M249
8 ΔTMM248-50 PGF---AAILAYTIG + -   M249
9 ΔFTMM247-50 PG----AAILAYTIG - - 5aa 5aa
  1. The primary DV2 mutant focus assay screen determined which mutants were able to produce foci of infections in Vero and A. Albopictus C6/36 cells. (+) indicates the presence of foci and (-) indicates no foci were observed. AA; amino acid.