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Figure 4 | Virology Journal

Figure 4

From: Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype

Figure 4

Electron micrograph thin sections of WT DV2, DV2ΔGVII, DV2ΔLIG viruses in the C6/36 line of mosquito cells (column 1; frames A, C, & E) and the mammalian Vero cell line (column 2; frames B, D, & F). WT DV2 virus particles are seen in large paracrystalline structures in infected mosquito cells (Figure 4A-arrows and inset). In infected mammalian cells, WT virus particles are associated with the plasma cell membrane (Figure 4B-arrows, inset). Mosquito C6/36 cells infected with the DV2ΔGVII mutant display similar amounts of virus particles (Figure 4C-arrows, inset) as compared to that observed in the WT virus infected mosquito cells. However, in mammalian cells infected with DV2ΔGVII (Figure 4D) only the presence of nucleocapsids in the cytoplasm and associated with internal membranes were detected in the thin sections (arrowheads and magnified in the inset). Similarly, DV2ΔLIG virus particles were observed in the cytoplasm of infected C6/36 mosquito cells although fewer particles were observed (Figure 4E-arrowheads) while only nucleocapsids were detected in the cytoplasm of the infected mammalian cells (Figure 4F-arrowheads, inset). Bars are 500 mM and the insets are 2X magnified from the original.

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