Figure 3

Western blot analysis of DV2 host-range mutants. A 4-12% bis-tris gradient gel was loaded with 5 μl of iodixanol-gradient purified WT or 25 μl of mutant DV2 virus purified from the supernatant of infected mosquito C7-10 cells. Proteins were stained using a polyclonal serum raised against whole DV2. The size of the molecular weight markers is indicated on the right in kDa. Lane 1, WT DV2; Lane 2, DV2ΔGVII; Lane 3, DV2ΔLIG. E; E protein; preM, preM protein.