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Figure 2 | Virology Journal

Figure 2

From: Analysis of a viral metagenomic library from 200 m depth in Monterey Bay, California constructed by direct shotgun cloning

Figure 2

Buoyant density and genome size distribution of virus-like particles used to construct the library. Panel A: The density (open circles) of each fraction collected from an equilibrium buoyant density gradient is plotted along with the concentration of viruses (filled triangles) in the fraction. Fractions 1 through 4 from the top of the gradient were harvested and pooled to construct the library. Error bars for density represent the standard deviation from triplicate measurements. Error bars for viral abundance represent the standard deviation of counts from multiple fields of a single filter for each fraction. Panel B: Image of DNA from the purified viral fraction separated by pulsed-field gel electrophoresis. Lane 1, Size standard (5 kb ladder); Lane 2, Size standard (Lambda DNA ladder); Lane 3, pooled viruses loaded after simple heat shock in TE to release DNA; Lane 4, DNA from purified viruses after organic extraction with phenol-chloroform.

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