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Figure 4 | Virology Journal

Figure 4

From: Rotavirus NSP4: Cell type-dependent transport kinetics to the exofacial plasma membrane and release from intact infected cells

Figure 4

EndoH sensitivity of NSP4 localized to the exofacial surface. MDCK and HT29.F8 cells were infected with RV SA11.4F for 7 h (MOI = 2) biotinylated at 4°C, lysed, and treated with EndoH prior to Western blot analyses. Lysates and precipitated surface proteins from MDCK cells at 7 hpi and from HT29.F8 cells at 7 and 4 hpi are shown. Lane: 1 = MDCK lysates, pre EndoH treatment, 7 hpi. 2 = MDCK lysates, mock EndoH treatment, 7 hpi. 3 = MDCK lysates, EndoH treated, 7 hpi. 4 = MDCK PM (streptavidin pull-down), pre Endo H treatment, 7 hpi. 5 = MDCK PM, mock EndoH treatment, 7 hpi. 6 = MDCK PM, Endo H treated, 7 hpi. 7 = HT29.F8 lysates, pre EndoH treatment, 7 hpi. 8 = HT29.F8 lysates, mock EndoH treatment, 7 hpi. 9 = HT29.F8 lysates, EndoH treated, 7 hpi. 10 = HT29.F8 PM (streptavidin pull-down), pre EndoH treatment, 7 hpi. 11 = HT29.F8 PM, mock EndoH treatment, 7 hpi. 12 = HT29.F8 PM, EndoH treated, 7 hpi. 13 = HT29.F8 lysates, pre EndoH treatment, 4 hpi. 14 = HT29.F8 lysates, mock EndoH treatment, 4 hpi. 15 = HT29.F8 lysates, EndoH treated, 4 hpi. 16 = HT29.F8 PM, pre EndoH treatment, 4 hpi. 17 = HT29.F8 PM, mock EndoH treatment, 4 hpi. 18 = HT29.F8 PM, EndoH treated, 4 hpi.

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