Figure 4From: Rotavirus NSP4: Cell type-dependent transport kinetics to the exofacial plasma membrane and release from intact infected cellsEndoH sensitivity of NSP4 localized to the exofacial surface. MDCK and HT29.F8 cells were infected with RV SA11.4F for 7 h (MOI = 2) biotinylated at 4°C, lysed, and treated with EndoH prior to Western blot analyses. Lysates and precipitated surface proteins from MDCK cells at 7 hpi and from HT29.F8 cells at 7 and 4 hpi are shown. Lane: 1 = MDCK lysates, pre EndoH treatment, 7 hpi. 2 = MDCK lysates, mock EndoH treatment, 7 hpi. 3 = MDCK lysates, EndoH treated, 7 hpi. 4 = MDCK PM (streptavidin pull-down), pre Endo H treatment, 7 hpi. 5 = MDCK PM, mock EndoH treatment, 7 hpi. 6 = MDCK PM, Endo H treated, 7 hpi. 7 = HT29.F8 lysates, pre EndoH treatment, 7 hpi. 8 = HT29.F8 lysates, mock EndoH treatment, 7 hpi. 9 = HT29.F8 lysates, EndoH treated, 7 hpi. 10 = HT29.F8 PM (streptavidin pull-down), pre EndoH treatment, 7 hpi. 11 = HT29.F8 PM, mock EndoH treatment, 7 hpi. 12 = HT29.F8 PM, EndoH treated, 7 hpi. 13 = HT29.F8 lysates, pre EndoH treatment, 4 hpi. 14 = HT29.F8 lysates, mock EndoH treatment, 4 hpi. 15 = HT29.F8 lysates, EndoH treated, 4 hpi. 16 = HT29.F8 PM, pre EndoH treatment, 4 hpi. 17 = HT29.F8 PM, mock EndoH treatment, 4 hpi. 18 = HT29.F8 PM, EndoH treated, 4 hpi.Back to article page