Figure 10
Confocal analyses of RV-infected HT29.F8 cells at 7 hpi. Panel A: Live HT29.F8 cells were surface biotinylated prior to fixation and permeabilization. Once the cells. were fixed and permeabilized, streptavidin-CY2 was utilized to label the biotinylated proteins on the exofacial PM (pseudo-colored red) and CY5-linked F(ab)2 prepared from anti-NSP4150-175 was added to label NSP4 (pseudo-colored green). The white, block arrow indicates an infected cell stained with anti-NSP4150-175-CY5-linked F(ab)2. The yellow arrows highlight the PM of the uninfected cells in which CY5-linked NSP4150-175 F(ab)2 is bound (green). Panel B is an enlargement of the white box in panel A and Panel C is the uninfected cell control that was labeled the same as the infected cells (panel A).