Figure 8

Morphogenesis of the constructed PRV SA215 strain (A to J). Virion adsorption and penetration (A, 35,000×) and formation and release of nucleocapsids into the cytoplasm (B, 30,000×) were observed. A large number of nucleocapsids aggregated and arrayed as pseudocrystals in the cell nucleus with insignificant enlargement of the nuclear membrane, and virion budding into the nuclear membrane were seen (C, 20,000×). Nucleocapsids budding through the inner membrane to obtain a primary envelope and budding out from the outer nuclear membrane to gain a secondary envelope, and loss of a primary envelope in the endoplasmic reticulum (D, 20,000×). Nucleocapsid release through the nuclear membrane and virions wrapped with tubular vesicles (E, 30,000×). Cytoplasmic nucleocapsids gaining envelopes through budding into the vesicles (F, 30,000×). Virion release (G, 30,000×). A large number of non-envelope capsids aggregated with electron-dense concomitant materials were found in the cytoplasm (H, 20,000×). Numerous nucleocapsids amassed in the inner or inside the nuclear membrane (I, 25,000×). Several envelopes from the nucleocapsids were observed in the nucleus (J, 15,000×)