Inhibition of lung serine proteases in mice: a potentially new approach to control influenza infection

Bahgat, Mahmoud M; Błazejewska, Paulina; Schughart, Klaus

doi:10.1186/1743-422X-8-27

Virology Journal

Table 1 Expression profiles of transcripts encoding lung proteases at various times after influenza infection of C57BL/6J and DBA/2J mice with PR8 virus

From: Inhibition of lung serine proteases in mice: a potentially new approach to control influenza infection

Gene				Relative quantification (2^-dct)/Days post infection
	Day 0			Day 1			Day 3			Day 6
	B6	DBA	P-value	B6	DBA	P-value	B6	DBA	P-value	B6	DBA	P-value
Ctsd	0.014 ± 0.0023	0.0054 ± 1.260E-06	<0.05	0.0077 ± 0.00018	0.0068 ± 0.0005	>0.05	0.0042 ± 0.00039	0.0082 ± 0.00028	<0.05	0.0027 ± 0.00046	0.0016 ± 2.192E-08	<0.05
Ctrl	0.1079 ± 0.0275	0.1705 ± 0.0581	>0.05	0.1031 ± 0.0179	0.1359 ± 0.0166	>0.05	0.1353 ± 0.054	0.1403 ± 0.03	>0.05	0.1018 ± 0.0046	0.1029 ± 0.0161	>0.05
Gzma	0.989 ± 0.0042	0.884 ± 0.012	<0.05	0.866 ± 0.0271	0.973 ± 0.0094	<0.05	0.9523 ± 0.0213	0.9874 ± 0.0071	>0.05	0.9941 ± 0.0016	0.9537 ± 0.0367	>0.05
Gzmb	0.0108 ± 0.0053	0.0390 ± 0.0139	<0.05	0.0095 ± 0.0041	0.0152 ± 0.0062	>0.05	0.0129 ± 0.0036	0.0085 ± 0.0023	>0.05	4.761 ± 0.0034	0.0285 ± 1.671	<0.05
Gzmc	0.0134 ± 0.0004	0.0059 ± 0.0042	>0.05	0.004 ± 0.0002	0.2327 ± 0.0148	<0.05	0.004 ± 0.0012	0.258 ± 0.0114	<0.05	0.108 ± 0.0393	0.0285 ± 0.0034	<0.05
Gzmg	0.0066 ± 0.0007	0.0017 ± 8.538E-06	<0.05	0.0017 ± 0.0009	0.0044 ± 0.0012	>0.05	0.0036 ± 0.0002	0.0024 ± 0.0003	<0.05	0.0052 ± 0.0016	0.0027 ± 0.0003	>0.05
Gzmk	0.0079 ± 0.0021	0.0042 ± 0.0011	>0.05	0.0036 ± 0.0006	0.0026 ± 0.0003	>0.05	0.004 ± 0.00043	0.003 ± 0.00018	<0.05	0.0097 ± 0.0009	0.0062 ± 0.0004	<0.05
Mmp 1a	0.0294 ± 0.0047	0.0057 ± 8.380E-05	<0.05	0.01165 ± 0.0025	0.0067 ± 0.0029	>0.05	0.0061 ± 0.0004	0.0084 ± 0.0016	>0.05	0.0062 ± 0.0021	0.0197 ± 0.0044	<0.05
Mmp 1b	0.0097 ± 0.0007	0.0056 ± 0.0002	<0.05	0.0071 ± 0.0011	0.0055 ± 8.095E-05	>0.05	0.0061 ± 0.0005	0.0035 ± 0.0007	<0.05	0.005 ± 0.0004	0.003 ± 0.0008	<0.05
Mmp 2	0.00012 ± 7.071E-06	0.0002 ± 9.441E-05	>0.05	0.00016 ± 4.543E-05	0.00019 ± 1.790E-05	>0.05	7.7 E-05 ± 1.90E-05	0.00011 ± 1.579E-06	<0.05	0.00016 ± 3.126E-05	0.00011 ± 1.331E-05	>0.05
Mmp 8	0.00055 ± 0.00026	0.00021 ± 3.525E-05	>0.05	0.0003 ± 4.16E-05	0.00038 ± 0.00018	>0.05	0.00046 ± 0.0002	0.00176 ± 6.903E-05	<0.05	0.00122 ± 0.0005	0.00124 ± 0.0009	>0.05
Mmp 9	0.00116 ± 0.0002	0.00036 ± 0.0004	<0.05	0.003 ± 8.889E-05	0.0013 ± 0.0001	<0.05	0.00043 ± 0.00015	0.00172 ± 3.380E-05	<0.05	0.0006 ± 0.0007	0.0008 ± 0.0005	>0.05
Elane	0.1555 ± 0.0198	0.2328 ± 0.0349	<0.05	0.2083 ± 0.023	0.1490 ± 0.040	>0.05	0.3728 ± 0.0820	0.2061 ± 0.039	<0.05	0.1838 ± 0.015	0.1706 ± 0.034	>0.05
Tmprss2	0.02647 ± 0.0181	0.03916 ± 0.0344	>0.05	0.00682 ± 0.01076	0.03398 ± 0.0273	>0.05	0.00305 ± 0.00343	0.02584 ± 0.03484	>0.05	0.00521 ± 0.00066	0.00232 ± 0.0017	>0.05
Tmprss4	0.2649 ± 0.1166	0.4376 ± 0.1963	>0.05	0.1990 ± 0.2406	0.7521 ± 0.3214	>0.05	0.2988 ± 0.08959	0.3265 ± 0.1309	>0.05	0.4279 ± 0.0147	0.03122 ± 0.0139	<0.05
Tpsg1	0.1033 ± 0.0492	0.06162 ± 0.0117	>0.05	0.04691 ± 0.05	0.0441 ± 0. 0.05	>0.05	0.1455 ± 0.0917	0.05375 ± 0.0187	>0.05	0.00418 ± 0.0017	0.0281 ± 0.0089	<0.05

Each relative quantification value represents the mean of three independent measurements using RNA from three individual C57Bl/6J or DBA/2J mice at the indicated time points post infection with the A/Puerto Rico/8/34 (H1N1; PR8) influenza virus. The difference in the levels of expression of various protease genes among the 2 mouse strains was considered significant when P-value was < 0.05.

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ISSN: 1743-422X

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