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Figure 3 | Virology Journal

Figure 3

From: Distinct different expression of Th17 and Th9 cells in coxsackie virus B3-induced mice viral myocarditis

Figure 3

The levels of cardiac IL-17 and IL-9 mRNA were detected by PCR. A. Representative images showing semi-quantitative reverse transcription-PCR products for IL-17 mRNA transcription in VMC group. Lane M, 50-bp marker ladder;Lanes1-7, Day 0,7,14,21,28,35,42 subgroups. B.Collective analyses of results from all groups for IL-17 mRNA. The products were normalized versus β-actin mRNA band intensities. C. The percentages of cardiac IL-9 mRNA were detected by nested-PCR. Representative images showing PCR products of the samples. Lane M,100-bp marker ladder;Lanes1,2, samples of control group; Lanes 3-16, samples of different time point in VMC groups; Lanes 3,4, Day0; Lanes 5,6, Day7; Lanes 7,8, Day14; Lanes9,10, Day21; Lanes11,12, Day28; Lanes13,14, Day35; Lanes 15,16, Day42. Lane17, sterile water were amplified as the negative control. n = 5 mice for per control subgroup, n = 6-10 for per VMC subgroup. Values are expressed as mean ± SD. *, p < 0.01 vs. control subgroups sacrificed on the same point time; #, p < 0.05 vs. other VMC subgroups;Δ, p < 0.01 vs. Day14,21,28,35,42 subgroups of the VMC group.

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