The frequencies of Th17 and Th9 cells were investigated by flow cytometry. A. Representative pictures for Th17 cells, and Th9 cells in VMC group. Numbers in upper left quadrants and lower right quadrants indicate the separate percentages of CD4+IL-17+IL-9- Th17 cells and CD4+IL-9+IL-17- Th9 cells, respectively. B,C. The results of statistical analysis for the alteration of Th17 and Th9 cells in different groups. n = 5 mice for per control subgroup, n = 6-10 for per VMC subgroup. Values are expressed as mean ± SD. *, p < 0.05 vs. control subgroups sacrificed on the same time point; #, p < 0.01 vs. other VMC subgroups; Δ, p < 0.01 vs. Day14,21,28,35,42 subgroups of VMC.