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Figure 7 | Virology Journal

Figure 7

From: Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene

Figure 7

Transcriptional analysis of DEV UL55 gene in infected cells in vitro. The transcriptional analysis of DEV UL55 in infected DEFs were performed by qRT-PCR. The transcriptional expression of DEV UL55 gene was normalized to a reference gene(β-actin) and relative to NTC control. The average relative content of the DEV UL55 gene transcripts were calculated at 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12, 16, 24, 36, 48, and 60 h post-infection using iCycler IQ 5.

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