Figure 3From: Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 geneThe amplication curves of DEV UL55 gene and β-actin by qRT-PCR. A, Ten-fold dilutions of standard pMD18-T/UL55 ranging from 1.0 × 10-7 to 1.0 × 10-1 copies/reaction were used for amplification, as indicated in the x-axis, whereas the corresponding Ct values are presented on the y-axis. B, Ten-fold dilutions of standard pMD18-T/β-actin ranging from 1.0 × 10-8 to 1.0 × 10-4 copies/reaction were used for amplification, as indicated in the x-axis, whereas the corresponding Ct values are presented on the y-axis.Back to article page