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Figure 1 | Virology Journal

Figure 1

From: Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene

Figure 1

Cloning of DEV UL55 gene and identification of recombinant plasmid pMD18-T/UL55 by restriction enzyme digestion. A, The amplification of DEV UL55 gene by conventional PCR using primers P1, P2. Lane 1, the amplified product of DEV UL55 gene; M, DNA marker(DL2000). B, Identification of recombinant plasmid pMD18-T/UL55 by restriction enzyme digestion. Lane 1, the recombinant plasmid pMD18-T/UL55 were digested with BamH I and Xho I; Lane 2, the PCR product of the recombinant plasmid pMD18-T/UL55; M1, DNA marker III; M2, DNA marker(DL2000).

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