Skip to main content

Table 1 Oligonucleotide primers used in this study

From: Co-expression of Ubiquitin gene and capsid protein gene enhances the potency of DNA immunization of PCV2 in mice

Gene

Primers

Sequences of primers(5'→3')

Length

Anneal tem

ORF2

Fw

AATCTCGAG GCCACCATGACGTATCCAAGG

717 bp

56°C

 

Rv

ATTAGATCT TTATTCATTAAGGGTTAAGTG

  

ub-linker

fw1

gga gagctc gccaccatgcagatcttcgtg

270 bp

56°C

 

rv1

gccgccgccgctgccgccgccgccgctgccgccgccgcc tcttcccctcaagcg

  

linker-ORF2

fw2

ggcggcggcggcagcggcggcggcggcagcggcggcggc ATGACGTATCCAAGG

790 bp

56°C

 

rv2

ctcgag TTATTCATTAAGGGTTAAGTGGGGGG

  

U-ORF2

fw1

gga gagctc gccaccatgcagatcttcgtg

1030 bp

56°C

 

rv2

ctcgag TTATTCATTAAGGGTTAAGTGGGGGG

  
  1. Note: Italic denotes restriction enzyme site; underline denotes linker; TTA denotes stop.
Back to article page

Virology Journal

ISSN: 1743-422X