Identification of CD8+ cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice

Table 1 Primers designed for PCR

primer	Sequence (5'-3')	Note
M-F	CCCAAGCTT AGC ATG GGGTCGTCTCTAGA	Hind III
M-R	CCGCTCGAG CTA TTTGGCATATTTGACA	Xho I
Ub-F	TCAGGATCC GCCGCCGCC ATG CAGATTTTCGTG	BamH I
Ub-R	GGAGCCTGGAGAAGCACCTCTCAGGCGAAGGAC
Truncated-M-F	CCGGAATTC ATGGGAGTGTACTCGGCCATAG	EcoR I
Truncated-M-R	CCGCTCGAG CTATTTGGCATATTTGAC	Xho I
Fusion-M-Ub-F	TCTCCAGGCTCC ATGGGGTCGTCTCTA	Linker
Fusion-M-Ub-R	GGAGCCTGGAGA AGCACCTCTCAGGCGAAGGAC	Linker
pSC11-M-F	ATAGTCGAC GCCGCCGCC ATG GGGTCGTCTCTAG	Sal I
pSC11-M-R	GCCGTCGAC CTA TTTGGCATATTTGACAAGGTTTAC	Sal I
P7.5-F	GCACGGTAAGGAAGTAGAAT

Note: The bold letter of primers are start or stop codons; the underlined sequence of primers represents the Kozak motif or the linker sequence.

ISSN: 1743-422X