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Figure 1 | Virology Journal

Figure 1

From: Identification of CD8+ cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice

Figure 1

A representative response was obtained from one mouse on 3 day after boosting with rWR-PRRSV-M, and two M protein peptides stimulate IFNγ+ production in CD8+ T cells of immunized BALB/c mice by ICS. Splenocytes from immunized BALB/c mice were (A) stimulated with PMA and ionomycin: 20.23%, (B) left unstimulated: 0.24%, (C) stimulated with an irrelevant peptide: 0.03%, (D) stimulated with the peptide "K93F ITSRCRL": 4.97%, or (E) stimulated with the peptide "F57G YMTFVHF": 4.12%, and were stained with antibodies to identify IFNγ production in CD8+ T cells by flow cytometry. The % in each data plot represents the percentage of CD8+ T cells that secreted IFNγ.

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Virology Journal

ISSN: 1743-422X