Construction of recombinant virus and production of the E protein. (A) Schematic representation of the construction of the recombinant virus vSynYFE, showing homologous recombination between the plasmid pSynYFE and the lacZ region (polh locus) in the virus vSynVI-gal. (B) SDS-PAGE analysis of E protein expression in insect cells. Lane 1, molecular weight marker (BenchMark™ Prestained Protein Ladder - Invitrogen); Lane 2, Tn5B cells mock infected; Lane 3, Tn5B cells infected with wild type AcMNPV; Lane 4, Tn5B cells infected with vSynVI-gal; Lane 5, Tn5B cells infected with Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV); Lanes 6, 7 and 8 Tn5B cells infected with a recombinant AcMNPV containing the ScathL gene from Sarcophaga peregrina (vSynScathL) and Lane 9, Tn5B cells infected with the recombinant vSynYFE. Arrow indicate a major polypeptide band of around 54 kDa in vSynYFE-infected Tn5B cells.