Figure 2From: Yellow fever virus envelope protein expressed in insect cells is capable of syncytium formation in lepidopteran cells and could be used for immunodetection of YFV in human seraConstruction of recombinant virus and production of the E protein. (A) Schematic representation of the construction of the recombinant virus vSynYFE, showing homologous recombination between the plasmid pSynYFE and the lacZ region (polh locus) in the virus vSynVI-gal. (B) SDS-PAGE analysis of E protein expression in insect cells. Lane 1, molecular weight marker (BenchMarkâ„¢ Prestained Protein Ladder - Invitrogen); Lane 2, Tn5B cells mock infected; Lane 3, Tn5B cells infected with wild type AcMNPV; Lane 4, Tn5B cells infected with vSynVI-gal; Lane 5, Tn5B cells infected with Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV); Lanes 6, 7 and 8 Tn5B cells infected with a recombinant AcMNPV containing the ScathL gene from Sarcophaga peregrina (vSynScathL) and Lane 9, Tn5B cells infected with the recombinant vSynYFE. Arrow indicate a major polypeptide band of around 54 kDa in vSynYFE-infected Tn5B cells.Back to article page