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Figure 1 | Virology Journal

Figure 1

From: Yellow fever virus envelope protein expressed in insect cells is capable of syncytium formation in lepidopteran cells and could be used for immunodetection of YFV in human sera

Figure 1

Amplification of the envelope gene (env). Amplification of the Yellow Fever env gene by RT-PCR. (A) Agarose gel (0.8%) eletrophoresis showing the RT-PCR amplification product (lane 2) using the oligonucleotides YFE2460R e YFE878F. Lane 1 shows the molecular mass marker, 1 Kb plus DNA ladder (Invitrogen). (B) Diagram of the Yellow Fever genome (Genebank accession # YFU17066 ) showing the main ORF (polyprotein ORF) and the position of the structural (Cap, pM and E) and non-structural (NS1, NS2a, NS2b, NS3, NS4a and NS5) proteins. The position of the oligonucleotides YFE2460R e YFE878F, used for the amplification reaction are also shown.

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Virology Journal

ISSN: 1743-422X