Table 1 Primer sets used to clone NoV capsid regions, HRV VP7, and HAV VP1/P2A.

NoV GI Capsid

GI-FIM; CTGCCCGAATTYGTAAATGATGAT

5342-5365^a

Onestep RT-PCR

GI-RIM; CCAACCCARCCATTRTACATYTG

5649-5671^a

Onestep RT-PCR and Semi-nested PCR

GI-F2; ATGATGATGGCGTCTAAGGACGC

5358-5380^a

Seminested PCR

NoV GII Capsid

GII-FIM; GGGAGGGCGATCGCAATCT

5049-5067^b

Onestep RT-PCR

GII-RIM; CCRCCIGCATRICCRTTRTACAT

5367-5389^b

Onestep RT-PCR and Semi-nested PCR

GII-F3M; TTGTGAATGAAGATGGCGTCGART

5079-5102^b

Semi-nested PCR

HRV VP7

ddrv-1; GGCGCC GCTCYTTTTRATGTATGGTATTGAATTACCAC

6-38^c

Onestep RT-PCR

ddrv-2; GGCGCC CTTTAAAATANAYDGADCCWRTYGGCCA

346-373^c

Onestep RT-PCR

HAV VP1/P2A

HAV-1F; GGCGCC ATTCAGATTAGACTGCCTTGGTA

2789-2811^d

Onestep RT-PCR

HAV-1R; GGCGCC AGTAAAAACTCCAGCATCCATTTC

3365-3388^d

HAV-2F; CTATTCAGATTGCAAATTACAAT

2886-2908^d

Semi-nested PCR

HAV-2R; AACTTCATTATTTCATGCTCCT

3258-3279^d