Figure 4

Antiviral effect of chloroform extract of Solanum nigrum against HCV NS3 protease. Huh-7 cells were transfected with 0.5 μg of constructed HCV NS3 protease vector in the presence and absence of SN and interferon for 24 48 hrs. Cells were harvested and relative RNA determinations were carried out using semi-quantitative RT-PCR. The results demonstrated that SN and interferon inhibit HCV NS3 expression while the expression of GAPDH remained constant.