Figure 10

Expression of Programmed Death 1 (PD-1) on CD4 negative T cell from mice. The CD4+ T cells from infected and control mice were purified at different hours post infection (PI). The cells were stained with anti mouse PD-1 antibody conjugated with phycoerythrin-Cy7 (PE-Cy7). The cells negative for CD4 receptors were gated and the percentage of CD4 negative T cells expressed the PD-1 were calculated by formula mentioned in methods. The biexponential transformation of dot plot represents the percentage of CD4- population which showed significant changes in expression of PD-1 at different hour post infection (PI).^b p < 0.01