Figure 3

The optimal expression condition of pET32a(+)/UL55 protein. A. The determination of the optimal final concentration of IPTG. Lanes 1, 2, 3, 4, 5 and 6 represented the final concentrations of IPTG were 1.0 mM, 0.8 mM, 0.6 mM, 0.4 mM, 0.2 mM and 0.1 mM respectively. B. The determination of the optimal induced temperature. Lanes 1, 2 and 3 represented the induction temperatures were 30°C, 37°C and 25°C, respectively. C. The determination of the optimal duration of IPTG. Lanes 1, 2, 3, 4 and 5 represented the durations of IPTG were 4.0 h, 5.0 h, 6.0 h, 7.0 h and 8.0 h, respectively.