Figure 10

Intracellular localization of DEV pUL55 in mock-infected cells and DEV-infected cells at 5.5 h post-infection. A. Mock-infected cells were fixed; B-C. DEV-infected cells were fixed at 5.5 h post-infection; The sample B was incubated with rabbit pre-immune serum while samples A and C were incubated with anti-pUL55 serum and subsequently stained with fluorescein isothiocyanate(FITC)-conjugated secondary antibody. Nuclei were counterstained with DAPI(blue). The merged fluorescence microscopy image of DEF are shown in panels A-C with high magnification (600×).