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Figure 4 | Virology Journal

Figure 4

From: Infection by agnoprotein-negative mutants of polyomavirus JC and SV40 results in the release of virions that are mostly deficient in DNA content

Figure 4

Analysis of late capsid protein (VP1) expression in PHFG cells transfected/infected with either JCV Mad-1 WT or its Pt mutant genome. (A) Nuclear extracts were prepared from PHFG cells transfected either with JCV Mad-1 WT or Pt mutant genome and analyzed by Western blot for VP1 expression. (B) CV-1 cells were transfected either with SV40(776) WT or the Pt mutant genome; and the nuclear extracts were prepared at the days indicated and analyzed by Western blot for VP1 expression. In lane 1 in each panel, nuclear extracts prepared from untransfected cells were loaded as negative control (- Cont.). Blots for panel A and B were also analyzed for Lamin A and C expression to demonstrate equal loading of nuclear proteins onto the gels.

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