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Figure 7 | Virology Journal

Figure 7

From: Mutation of the elongin C binding domain of human respiratory syncytial virus non-structural protein 1 (NS1) results in degradation of NS1 and attenuation of the virus

Figure 7

The accumulation of mutated NS1 protein within lysosomes does not involve autophagy. Vero cells were infected with either NS1F, NS1F-ELCmut, or mock infected with media, and cells were lysed in RIPA buffer 24 h and 48 h p.i. Equivalent amounts of total protein were separated by SDS-PAGE and analysed by Western blot. The active (LC3-II) and constitutive (LC3-I) forms of LC3 were detected using anti-LC3B polyclonal antibody. NS1 was detected using anti-FLAG monoclonal antibody. Representative of three independent experiments.

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Virology Journal

ISSN: 1743-422X