Figure 6

Different PDZ domains have different binding characteristics for the same ligand. Upper panel. The cartoon shows the GST-NTMAGI-1 and GST-CTMAGI-1 fusion proteins. Also shown is the GST-Scrib4PDZ fusion protein, which comprises all four of the PDZ domains of Scribble, aligned with the full-length protein for reference. Lower panel. A GST pulldown assay was performed with these fusion proteins plus the GST alone and GST-DLG as negative and positive controls, respectively. They were incubated as before with the in vitro translated Avian, Human and non-PDZ binding mutant NS1 proteins. Human papillomavirus type 18 E6 was included for comparison.