Figure 1From: Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteinsHuman and avian type NS1 protens differ in PDZ-binding activity. A GST-pulldown assay was performed using bacterially expressed GST and GST-Dlg, with GST-p53 as a non-PDZ-containing control. These were incubated with in vitro translated radiolabelled Avian type (A) or Human type (H) NS1 proteins. After extensive washing the bound proteins were eluted and analysed by SDS-PAGE and autoradiography (upper panel). The lower panel shows the Coomassie-stained gel; the GST fusion proteins are arrowed.Back to article page