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Figure 3 | Virology Journal

Figure 3

From: Inhibition of Hazara nairovirus replication by small interfering RNAs and their combination with ribavirin

Figure 3

Antiviral activities of siS1 and siS2 transfected before and after HAZV infection. A) A549 cells were transfected with 100 nM of siNT (siRNA negative control), siS1 or siS2, 24 hrs, 48 hrs or 72 hrs before HAZV infection (MOI 0.1). B) Cells were infected with HAZV at a MOI of 0.01 and then transfected with 100 nM of siNT, siS1 or siS2, 1 hr, 8 hrs or 24 hrs post-infection. Viral titers were determined 48 hrs post-infection. Results are shown as a ratio between virus titer obtained in siRNAs treated wells and titer determined in siNT transfected cells. Errors bars represent the standard deviation (SD) of the means for at least two independent experiments done in quadruplicates. * Significant differences compared to the siNT control: Student's t-test; P < 0.05.

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