Inhibition of HAZV replication by segment-specific siRNAs in A549 cells. A) Cells were transfected with 100 nM of the different siS (siS1 to siS4), B) siM (siM1 to siM4) or C) siL (siL1 to siL4). Twenty four hours post-transfection, cells were infected with HAZV at a MOI of 0.1. Viral titers were determined 48 hrs post-infection as described in the materials and methods section. Results are expressed as a percentage of average foci counts in siRNAs treated cells to that in siNT (siRNA negative control) transfected cells. Errors bars represent the standard deviation (SD) of the means for at least two independent experiments performed in quadruplicates. * Significant differences compared to the siNT control: Student's t-test; P < 0.05.