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Figure 3 | Virology Journal

Figure 3

From: Novel viral vectors utilizing intron splice-switching to activate genome rescue, expression and replication in targeted cells

Figure 3

Effects of insertion of aberrantly spliced introns into SFV DNA/RNA layered replicon vectors. A. Schematic presentation of vectors A2 and A3 (Table 1). Products of correct splicing (left) represent mRNAs for functional replicase of SFV and for the luciferase marker; the subsequent replication of these RNAs by SFV replicase results in template amplification and transcription of SG-mRNA used for EGFP expression. mRNAs resulting from aberrant splicing (right) express only P123 polyprotein. B. Luciferase activities in HeLa cells at 24 h after transfection with vectors A1, A2 and A3 are represented on the vertical axis; luciferase activities in cells transfected with A1 vectors were taken as 100%. Left panel, results for DNA/RNA layered replicon vectors; right panel, results for DNA/RNA layered replicon vectors containing FS mutation. C. Luciferase activities in HeLa cells at 24 h after transfection with vectors B0, C0, D0 and E0 are represented on the vertical axis; luciferase activities in cells transfected with B0 vectors were taken as 100%. Left panel, results for DNA/RNA layered replicon vectors; right panel, results for DNA/RNA layered replicon vectors containing FS mutation. D. Luciferase activities in HeLa cells at 24 h after transfection with vectors B1, C2, D2 and E3 are represented on the vertical axis; luciferase activities in cells transfected with B1 vectors were taken as 100%. Left panel, results for DNA/RNA layered vectors; right panel, results for DNA/RNA layered vectors containing FS mutation. Error bars on panels B-D represent standard deviations; experiments were repeated three times with similar results.

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