P6 is able to recruit P9-1 to VLS in onion epidermal cells. (A) Subcellular localization of P9-1 fused with GFP or DsRed2. P9-1-GFP and DsRed-P9-1 were distributed diffusely in the onion cells and were unable to form inclusion bodies. (B) Co-expression of P6-GFP and DsRed-P9-1 in onion epidermal cells. Detection of green (lane a) and red (lane b) fluorescence was achieved with excitation at 488 nm and 543 nm, respectively; co-localization of green and red fluorescence is indicated in yellow (lane c); superposition of the green and red fluorescence images as well as the bright field image is shown on the right (lane d). The co-expression results indicate that P6 was able to relocate the distribution of P9-1, that both proteins were present exclusively in the discrete and punctate foci, and that expression of DsRed2 or GFP had no aberrant effects on DsRed-P9-1 or P6-GFP distribution. Bars, 50 μm.